nuff human neonatal foreskin fibroblasts Search Results


nuff-1 cells  (GlobalStem)
90
GlobalStem nuff-1 cells
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pmc04019081-71-0-6?v=GlobalStem
Average 90 stars, based on 1 article reviews
nuff-1 cells - by Bioz Stars, 2026-07
90/100 stars
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nuff3-rq human newborn foreskin fibroblasts gsc-3404  (GlobalStem)
90
GlobalStem nuff3-rq human newborn foreskin fibroblasts gsc-3404
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Nuff3 Rq Human Newborn Foreskin Fibroblasts Gsc 3404, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pmc06981130__41467_2019_14235_MOESM1_ESM-369-1-10?v=GlobalStem
Average 90 stars, based on 1 article reviews
nuff3-rq human newborn foreskin fibroblasts gsc-3404 - by Bioz Stars, 2026-07
90/100 stars
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newborn human foreskin fibroblast (nuff) cells  (GlobalStem)
90
GlobalStem newborn human foreskin fibroblast (nuff) cells
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Newborn Human Foreskin Fibroblast (Nuff) Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pmc04733842-135-6-12?v=GlobalStem
Average 90 stars, based on 1 article reviews
newborn human foreskin fibroblast (nuff) cells - by Bioz Stars, 2026-07
90/100 stars
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conditioned pluriton media from nuff cells  (GlobalStem)
90
GlobalStem conditioned pluriton media from nuff cells
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Conditioned Pluriton Media From Nuff Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pm26595768-603-13-17?v=GlobalStem
Average 90 stars, based on 1 article reviews
conditioned pluriton media from nuff cells - by Bioz Stars, 2026-07
90/100 stars
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nuff medium  (GlobalStem)
90
GlobalStem nuff medium
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Nuff Medium, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pmc03973712-131-19-10?v=GlobalStem
Average 90 stars, based on 1 article reviews
nuff medium - by Bioz Stars, 2026-07
90/100 stars
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mrc-5  (ATCC)
99
ATCC mrc-5
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/custom%40ccl-171%4032411622?v=ATCC
Average 99 stars, based on 1 article reviews
mrc-5 - by Bioz Stars, 2026-07
99/100 stars
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bj  (ATCC)
99
ATCC bj
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Bj, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/custom%40crl-2522%4031980631?v=ATCC
Average 99 stars, based on 1 article reviews
bj - by Bioz Stars, 2026-07
99/100 stars
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matrigel  (Corning Life Sciences)
90
Corning Life Sciences matrigel
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Matrigel, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pm36290384-100-27-28?v=Corning+Life+Sciences
Average 90 stars, based on 1 article reviews
matrigel - by Bioz Stars, 2026-07
90/100 stars
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thp-1  (ATCC)
99
ATCC thp-1
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Thp 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/custom%40tib-202%4036288299?v=ATCC
Average 99 stars, based on 1 article reviews
thp-1 - by Bioz Stars, 2026-07
99/100 stars
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mitomycin-c (4 × 10 6 )-treated newborn human foreskin fibroblast (nuff) cells  (GlobalStem)
90
GlobalStem mitomycin-c (4 × 10 6 )-treated newborn human foreskin fibroblast (nuff) cells
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Mitomycin C (4 × 10 6 ) Treated Newborn Human Foreskin Fibroblast (Nuff) Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pmc04733842-89-1-15?v=GlobalStem
Average 90 stars, based on 1 article reviews
mitomycin-c (4 × 10 6 )-treated newborn human foreskin fibroblast (nuff) cells - by Bioz Stars, 2026-07
90/100 stars
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tb40/e mcherry -us28-r129a-3xf  (GlobalStem)
90
GlobalStem tb40/e mcherry -us28-r129a-3xf
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Tb40/E Mcherry Us28 R129a 3xf, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pmc11460310-433-26-18?v=GlobalStem
Average 90 stars, based on 1 article reviews
tb40/e mcherry -us28-r129a-3xf - by Bioz Stars, 2026-07
90/100 stars
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fetal bovine serum (fbs  (Avantor)
90
Avantor fetal bovine serum (fbs
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Fetal Bovine Serum (Fbs, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuff+human+neonatal+foreskin+fibroblasts/pmc06662646-707-28-32?v=Avantor
Average 90 stars, based on 1 article reviews
fetal bovine serum (fbs - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung fibroblasts (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.

Journal: Journal of Virology

Article Title: Host MicroRNA Regulation of Human Cytomegalovirus Immediate Early Protein Translation Promotes Viral Latency

doi: 10.1128/JVI.00481-14

Figure Lengend Snippet: Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung fibroblasts (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.

Article Snippet: Primary newborn human fibroblasts (NUFF-1 cells; GlobalStem) or primary human embryonic lung fibroblasts (MRC5 cells) were maintained in Dulbecco modified Eagle medium (DMEM) supplemented with 10% FBS, 2 mM l -glutamine, 0.1 mM nonessential amino acids, and 100 U/ml each penicillin and streptomycin.

Techniques: Infection, Mutagenesis, Virus, Stable Transfection, Transduction, Control, Expressing, Modification, Immunofluorescence