nuff human neonatal foreskin fibroblasts Search Results


mrc-5  (ATCC)
99
ATCC mrc-5
Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrc-5 - by Bioz Stars, 2026-03
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nuff-1 cells  (GlobalStem)
90
GlobalStem nuff-1 cells
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuff3-rq human newborn foreskin fibroblasts gsc-3404  (GlobalStem)
90
GlobalStem nuff3-rq human newborn foreskin fibroblasts gsc-3404
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Nuff3 Rq Human Newborn Foreskin Fibroblasts Gsc 3404, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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newborn human foreskin fibroblast (nuff) cells  (GlobalStem)
90
GlobalStem newborn human foreskin fibroblast (nuff) cells
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Newborn Human Foreskin Fibroblast (Nuff) Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
newborn human foreskin fibroblast (nuff) cells - by Bioz Stars, 2026-03
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newborn human foreskin fibroblasts nuffs  (AMS Biotechnology)
86
AMS Biotechnology newborn human foreskin fibroblasts nuffs
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Newborn Human Foreskin Fibroblasts Nuffs, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
newborn human foreskin fibroblasts nuffs - by Bioz Stars, 2026-03
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conditioned pluriton media from nuff cells  (GlobalStem)
90
GlobalStem conditioned pluriton media from nuff cells
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Conditioned Pluriton Media From Nuff Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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conditioned pluriton media from nuff cells - by Bioz Stars, 2026-03
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neonatal human foreskin fibroblasts  (AMS Biotechnology)
86
AMS Biotechnology neonatal human foreskin fibroblasts
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Neonatal Human Foreskin Fibroblasts, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
neonatal human foreskin fibroblasts - by Bioz Stars, 2026-03
86/100 stars
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human fibroblasts  (AMS Biotechnology)
86
AMS Biotechnology human fibroblasts
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Human Fibroblasts, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
human fibroblasts - by Bioz Stars, 2026-03
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inactivated foreskin fibroblasts  (AMS Biotechnology)
86
AMS Biotechnology inactivated foreskin fibroblasts
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Inactivated Foreskin Fibroblasts, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inactivated foreskin fibroblasts/product/AMS Biotechnology
Average 86 stars, based on 1 article reviews
inactivated foreskin fibroblasts - by Bioz Stars, 2026-03
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nuff medium  (GlobalStem)
90
GlobalStem nuff medium
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Nuff Medium, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hipsc line  (AMS Biotechnology)
86
AMS Biotechnology hipsc line
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Hipsc Line, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytotune ips sendai reprogramming kit  (Thermo Fisher)
90
Thermo Fisher cytotune ips sendai reprogramming kit
Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung <t>fibroblasts</t> (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.
Cytotune Ips Sendai Reprogramming Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung fibroblasts (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.

Journal: Journal of Virology

Article Title: Host MicroRNA Regulation of Human Cytomegalovirus Immediate Early Protein Translation Promotes Viral Latency

doi: 10.1128/JVI.00481-14

Figure Lengend Snippet: Cellular hsa-miR-200 inhibits wild-type infection but not infection with an UL122 (IE2) 3′ UTR mutant virus. (A) Primary human embryonic lung fibroblasts (MRC5 cells) were stably transduced with either a control retrovirus or one that overexpressed the C1 cluster of the hsa-miR-200 family. In each cell type, levels of hsa-miR-200b (light gray bars) or hsa-miR-200c (dark gray bars) were assessed by qPCR. Samples were normalized to those of cellular RNU44 and analyzed in triplicate. (B, C) MRC5 cells stably transduced with a C1-expressing lentivirus (white bars) or an empty control (gray bars) were then infected with either wild-type FixBACgfp virus (B) or FixBACgfpIE2cisΔ virus (C) at a multiplicity of 0.5 PFU/cell for 4 days. The titer of cell-free virus was then determined by a modified immunofluorescence assay for IE1. Samples were analyzed in triplicate. (D) MRC5 cells transduced with either the C1-expressing or control lentivirus were infected with either wild-type FixBACgfp virus or FixBACgfpIE2cisΔ virus at a multiplicity of 1 PFU/cell. Cell lysates were harvested at the indicated time points (hpi, hours postinfection), and IE2 levels were assessed using a monoclonal antibody (clone 3A9). α-Tubulin was used as a control.

Article Snippet: Primary newborn human fibroblasts (NUFF-1 cells; GlobalStem) or primary human embryonic lung fibroblasts (MRC5 cells) were maintained in Dulbecco modified Eagle medium (DMEM) supplemented with 10% FBS, 2 mM l -glutamine, 0.1 mM nonessential amino acids, and 100 U/ml each penicillin and streptomycin.

Techniques: Infection, Mutagenesis, Virus, Stable Transfection, Transduction, Control, Expressing, Modification, Immunofluorescence